rat anti mouse cd3 primary antibody  (Bio-Rad)

Journal: ImmunoTargets and Therapy

Article Title: Enhancing Anti-CTLA-4 Antibody Delivery to the Brain Using Focused Ultrasound and Microbubbles

doi: 10.2147/ITT.S569804

Figure Lengend Snippet: Co-localisation of CD3 + cells and MHC class II + cells with anti-CTLA-4 antibody . ( A and B ) Immunofluorescence staining of CD3 + cells in long-pulse ultrasound treated brains ( A ) and RaSP-treated brains ( B ) of wild-type mice superimposed with anti-CTLA-4 antibody fluorescence and DAPI (nuclei staining). ( C and D ) Immunofluorescence staining of MHC class II + cells in long-pulse ( C ) or RaSP-treated brains ( D ) of wild-type mice superimposed with anti-CTLA-4 antibody fluorescence and DAPI (nuclei staining).

Article Snippet: T cells were stained using a rat anti-mouse CD3 primary antibody (1:200; clone: KT3, #MCA500G, Bio-Rad, CA, USA) with 1% (w/v) milk powder (Tesco, Hertfordshire, England) dissolved in TBS (#524750-1EA, EMD Millipore Corporation, Burlington, MA, USA) and a secondary Alexa Fluor 488 goat anti-rat IgG H&L secondary antibody (1:400; #ab150157, Abcam, Cambridge, England) with 1% (w/v) milk powder in TBS.

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: ImmunoTargets and Therapy

Article Title: Enhancing Anti-CTLA-4 Antibody Delivery to the Brain Using Focused Ultrasound and Microbubbles

doi: 10.2147/ITT.S569804

Figure Lengend Snippet: CD3 + and MHC class II + invasion in ultrasound-treated brain regions . Immunofluorescence staining shows differences in the invasion of CD3 + ( A – D ) and MHC class II + ( E – H ) cells into the ultrasound-treated brain regions ( A, C, E and G ) compared to control brain regions ( B, D, F and H ) using long-pulse ( A, B, E and F ) and RaSP ( C, D, G and H ) sequences. The total count of CD3 + ( I ) and MHC class II + ( J ) cells was determined in treated brain regions targeted with ultrasound at 0.71 MPa with both long pulse and rapid short-pulse sequences compared to control (untreated) regions. A significant increase in T cells ( I ) and MHC class II + cells ( J ) was observed in ultrasound-treated compared to control brain regions in long-pulse treated brains. Scale bars represent 200 µm. The plot shows mean ± SEM (n= 3); * p-value ≤ 0.05 (0.0021 ( I )) and *** p-value ≤ 0.0001 (0.0001 ( J )).

Article Snippet: T cells were stained using a rat anti-mouse CD3 primary antibody (1:200; clone: KT3, #MCA500G, Bio-Rad, CA, USA) with 1% (w/v) milk powder (Tesco, Hertfordshire, England) dissolved in TBS (#524750-1EA, EMD Millipore Corporation, Burlington, MA, USA) and a secondary Alexa Fluor 488 goat anti-rat IgG H&L secondary antibody (1:400; #ab150157, Abcam, Cambridge, England) with 1% (w/v) milk powder in TBS.

Techniques: Immunofluorescence, Staining, Control

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Representative immunofluorescence microscopic images of TIGIT, CD3, and CD56 in BC tissues and adjacent tissues.

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: Immunofluorescence

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Flow cytometry histograms of CD3 + and TIGIT + T cells in PBC patients ( A and B ) versus healthy controls ( C and D ).

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: Flow Cytometry

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Comparison of TIGIT expression between two groups and the sensitivity of TIGIT for the diagnosis of PBC. ( A ) TIGIT level (%) on CD3 + T cells in PBC patients versus healthy controls. ( B ) The ROC curve drawn based on the percentage of TIGIT in peripheral blood T cells of PBC patients.

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: Comparison, Expressing, Biomarker Discovery

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Correlation Between TIGIT Level on Peripheral Blood T Cells and Clinicopathological Features in PBC Patients

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques:

Journal: Aging (Albany NY)

Article Title: p21 facilitates chronic lung inflammation via epithelial and endothelial cells

doi: 10.18632/aging.204622

Figure Lengend Snippet: p21 -/- decreases chronic inflammatory responses and iBALT formation caused by exposure to aerosolized LPS. WT and p21 -/- mice were exposed to either PBS or aerosolized LPS (0.5 mg/ml), 3 times a week for 10 weeks. ( A – F ) At 48 hours following the last LPS exposure, whole lungs were dissociated into single cell suspensions and analyzed by flow cytometry to determine: ( A ) numbers of immune cells (CD45+), ( B ) numbers of neutrophils (CD45+/Ly6G+/CD11b+), ( C ) numbers of interstitial macrophages (CD45+/CD11c+/SiglecF-/CD11b+/CD24+), ( D ) numbers of CD3+ T cells (CD45+/CD3+), ( E ) numbers of CD4+ T cells (CD45+/CD3+/CD4+), and ( F ) numbers of CD8+ T cells (CD45+/CD3+/CD8+). ( G ) mRNA expression levels of the indicated SASP factors in the mice lungs. ( H ) Representative images of lungs stained for CD3+ (red) and B220 (green) depict accumulation of iBALTs in LPS exposed mice. Scale bar represents 200μm. ( I , J ) Numbers ( I ) and sizes ( J ) of iBALTs in the lungs of mice exposed to aerosolized LPS. Data information: Data were analyzed using one-way ANOVA, *P<0.05. **<0.005. ***P<0.0005 ( A – G ), and by Student’s t-test, *p<0.05, **p<0.01, and ***p<0.005 ( I , J ). Data represent mean ±SEM ( A – F , n=9-12; G , n=3-6; H – J ; n=4-6 independent repeats).

Article Snippet: Primary antibodies recognizing CD3 (1:50, MCA500G, Bio-Rad), CD45R (#14-0452-82, eBioscience, 1:50), cleaved caspase 3 (1:50, #9661, Cell Signaling Technology) and p21 (1:50, 556431, BD Biosciences) were applied overnight at 4C.

Techniques: Flow Cytometry, Expressing, Staining

Journal: Aging (Albany NY)

Article Title: p21 facilitates chronic lung inflammation via epithelial and endothelial cells

doi: 10.18632/aging.204622

Figure Lengend Snippet: p21 -/- protects lung airways from chronic inflammatory responses triggered by chronic LPS exposure. WT and p21 -/- mice were exposed to either PBS or aerosolized LPS (0.5 mg/ml), 3 times a week for 10 weeks. ( A – F ) At 48 hours following the last LPS exposure BAL fluid was collected and flow cytometry was used to determine: ( A ) numbers of neutrophils (CD45+/Ly6G+/CD11b+), ( B ) numbers of alveolar macrophages (CD45+/CD11c+/SiglecF+), ( C ) numbers of B cells (CD45+/B220+), ( D ) numbers of CD3+ T cells (CD45+/CD3+), ( E ) numbers of CD4+ T cells (CD45+/CD3+/CD4+), and ( F ) numbers of CD8+ T cells (CD45+/CD3+/CD8+). Data information: Data were analyzed using one-way ANOVA. ***P<0.0005. Data represent mean ±SEM ( A – F , n=8-11 independent repeats).

Article Snippet: Primary antibodies recognizing CD3 (1:50, MCA500G, Bio-Rad), CD45R (#14-0452-82, eBioscience, 1:50), cleaved caspase 3 (1:50, #9661, Cell Signaling Technology) and p21 (1:50, 556431, BD Biosciences) were applied overnight at 4C.

Techniques: Flow Cytometry